PDE8B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-644

Novus Biologicals, part of Bio-Techne
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NB300-644
NB300-644-0.025mg
NB300-644-0.05mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Monkey

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all PDE8B Antibodies »

Product Specifications

Immunogen

Synthetic cyclic peptide common to all PDE8B variants.

Specificity

This has no cross-reactivity against PDE8A protein or any other PDE family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE8B Antibody

Western Blot: PDE8B Antibody [NB300-644]

Western Blot: PDE8B Antibody [NB300-644]

Western Blot: PDE8B Antibody [NB300-644] - Rat testis and heart membranes. Antibody (NB300-644) dilution 1:500. Molecular weight marker positions are marked on the left.
PDE8B Antibody [NB300-644] - Human ovarian medulla tissue. 1:2000 antibody dilution Image courtesy of Steven S. Petersen, MD, PHD Copenhagen University Hospital, Denmark.

Applications for PDE8B Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Immunoprecipitation and Western Blot Detects a band of approximately 98-100 kDa. Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE8B

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 5-prime,3-prime-cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been grouped into several families based on substrate affinities, inhibitor sensitivities, mode of regulation, and amino acid sequence homologies. The PDE8 family contains high-affinity cAMP-specific, IBMX (3-isobutyl-1-methyl-xanthine)-insensitive PDEs. All PDEs share a conserved C-terminal catalytic region and a variable N-terminal domain that presumably accounts for the distinctive regulatory properties unique to the individual families. PDE8B exhibits cAMP PDE activity which was not inhibited by various PDE inhibitors, including IBMX. The cAMP hydrolytic activity was unaffected by cGMP, and no cGMP PDE hydrolysis has been detected. Northern blot analysis indicated that the 4.2-kb PDE8B mRNA was expressed at the highest levels in thyroid, and at lower levels in brain, spinal cord, and placenta.

Long Name

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Alternate Names

HsPDE8B, PIG22

Entrez Gene IDs

8622 (Human); 218461 (Mouse); 309962 (Rat)

Gene Symbol

PDE8B

Additional PDE8B Products

Product Documents for PDE8B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDE8B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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