Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PDE9A Antibodies »

Product Specifications

Immunogen

Sequence from the C-terminal region of PDE9A

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE9A Antibody - BSA Free

Western Blot: PDE9A AntibodyBSA Free [NBP1-00641]

Western Blot: PDE9A AntibodyBSA Free [NBP1-00641]

Western Blot: PDE9A Antibody [NBP1-00641] - WB analysis of PDE9A in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: PDE9A Antibody - BSA Free [NBP1-00641]

Immunocytochemistry/ Immunofluorescence: PDE9A Antibody - BSA Free [NBP1-00641]

Immunocytochemistry/Immunofluorescence: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in NIH/3T3 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: PDE9A Antibody - BSA Free [NBP1-00641]

Immunohistochemistry: PDE9A Antibody - BSA Free [NBP1-00641]

Immunohistochemistry: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in mouse prostate using DAB with hematoxylin counterstain.

Applications for PDE9A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Immunoprecipitation

1:1000

Western Blot

1:1000
Application Notes
In Western Blot, a band can be seen at ~68 kDa. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE9A

Cyclic nucleotide phosphodiesterases (PDEs) are enzymes which catalyzes the hydrolysis of cyclic adenosine 30,50-monophosphate (cAMP) and/or cyclic guanosine 30,50-monophosphate (cGMP); and PDE9A (high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) is a PDE member which hydrolyzes the second messenger cGMP, an important regulator of several critical cellular physiological processes. PDE9A activity gets modulated by metal ions and it can bind 2 divalent metal cations per subunit - site 1 binds zinc ions, whereas, site 2 preferentially associates with magnesium and/or manganese ions. PDE9A is expressed in most of the solid tissues and because of its location as well as contribution to the regulation of steady-state cellular concentrations of cyclic nucleotides, PDE9A has been suggested as a potential candidate for diseases such as bipolar affective disorder and its overexpression has been linked to Down syndrome. Moreover, PDE9A deficient mice fed a high-fat diet have been reported to exhibit a reduced weight gain and fat mass compared with wild-type mice.

Long Name

High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A

Alternate Names

CGMP-specific 3'-5'-cyclic phosphodiesterase type 9, CGMP-specific 3'-5'-cyclic phosphodiesterase type 9, EC 3.1.4.1710phosphodiesterase PDE9A21, EC 3.1.4.35, FLJ90181, high affinity cGMP-specific 3'-5'-cyclic phosphodiesterase 9A, HSPDE9A2, phosphodiesterase 9A

Entrez Gene IDs

5152 (Human); 18585 (Mouse); 191569 (Rat)

Gene Symbol

PDE9A

Additional PDE9A Products

Product Documents for PDE9A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDE9A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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