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PDF Antibody (OTI3F3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73301

Novus Biologicals, part of Bio-Techne
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NBP2-73301
Conjugate
Catalog #

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3F3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human PDF (NP_071736) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDF Antibody (OTI3F3) - Azide and BSA Free

Western Blot: PDF Antibody (OTI3F3)Azide and BSA Free [NBP2-73301]

Western Blot: PDF Antibody (OTI3F3)Azide and BSA Free [NBP2-73301]

Western Blot: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.
Immunocytochemistry/ Immunofluorescence: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301]

Immunocytochemistry/ Immunofluorescence: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301]

Immunocytochemistry/Immunofluorescence: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF.
Flow Cytometry: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301]

Flow Cytometry: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301]

Flow Cytometry: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PDF antibody, and then analyzed by flow cytometry.

Applications for PDF Antibody (OTI3F3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDF

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq]

Alternate Names

EC 3.5.1.88, GDF15, MIC1, PDF1A, peptide deformylase (mitochondrial), peptide deformylase, mitochondrial, peptide deformylase-like protein, PLAB, Polypeptide deformylase

Gene Symbol

PDF

Additional PDF Products

Product Documents for PDF Antibody (OTI3F3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDF Antibody (OTI3F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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