PDK-1 Antibody (2E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00005170-M05

Novus Biologicals, part of Bio-Techne
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H00005170-M05
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDK-1 Antibodies »

Product Specifications

Immunogen

PDPK1 (NP_002604, 457 a.a. ~ 556 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PDK-1 Antibody (2E2)

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in rat brain.
Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in IMR-32.
Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05]

Western Blot: PDK-1 Antibody (2E2) [H00005170-M05] - Analysis of PDPK1 expression in transfected 293T cell line by PDPK1 monoclonal antibody (M05), clone 2E2. Lane 1: PDPK1 transfected lysate (Predicted MW: 48.2 KDa). Lane 2: Non-transfected lysate.

Applications for PDK-1 Antibody (2E2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDK-1

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Gene Symbol

PDPK1

Additional PDK-1 Products

Product Documents for PDK-1 Antibody (2E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDK-1 Antibody (2E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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