PDK-1 [p Tyr9] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23292
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-PDK-1 (Tyr-9) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 9 in human PDK-1. This sequence is highly conserved in rat and mouse PDK-1. [UniProt# O15530]
Modification
p Tyr9
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDK-1 (Tyr-9) peptide. The purified antibody detects a 65 kDa protein corresponding to the apparent molecular mass of PDK-1 on SDS-PAGE immunoblots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells. PDK1 (Tyr-9) reactivity was also observed in adult mouse brain.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for PDK-1 [p Tyr9] Antibody
![PDK-1 [p Tyr9] Antibody](https://resources.bio-techne.com/images/products/nbp3-23292_rabbit-pdk-1-p-tyr9-pab-19920231992816.jpg "Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] -")
Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] -
Western Blot: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Western blot image of A431 cells stimulated with pervanadate (lanes 1 and 3). The blots were then treated with akaline phosphatase (lanes 2 & 4) then probed with anti-PDK-1 (C-terminal region) mouse monoclonal antibody or anti-PDK-1 (Tyr-9) (NBP3-23292) rabbit polyclonal antibody.![PDK-1 [p Tyr9] Antibody](https://resources.bio-techne.com/images/products/nbp3-23292_rabbit-pdk-1-p-tyr9-pab-19920231856946.jpg "Immunocytochemistry/Immunofluorescence: PDK-1 [p Tyr9] Antibody [NBP3-23292] -")
Immunocytochemistry/Immunofluorescence: PDK-1 [p Tyr9] Antibody [NBP3-23292] -
Immunocytochemistry/Immunofluorescence: PDK-1 [p Tyr9] Antibody [NBP3-23292] - Immunocytochemical labeling of PDK-1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK-1 (C-terminal Region) (NBP3-23291), rabbit polyclonal PDK-1 (Tyr-9) (NBP3-23292), and PDK-1 (N-terminus) (NBP3-23290) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.Applications for PDK-1 [p Tyr9] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: PDK-1
Long Name
Phosphoinositide Dependent Kinase-1
Alternate Names
PDK1, PDPK1, PKB kinase
Gene Symbol
PDPK1
Additional PDK-1 Products
Product Documents for PDK-1 [p Tyr9] Antibody
Product Specific Notices for PDK-1 [p Tyr9] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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