PDK2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87307
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTN
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25991547).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDK2 Antibody
![Immunocytochemistry/ Immunofluorescence: PDK2 Antibody [NBP1-87307]](https://resources.bio-techne.com/images/products/PDK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87307-img0009.jpg "Immunocytochemistry/ Immunofluorescence: PDK2 Antibody [NBP1-87307]")
Immunocytochemistry/ Immunofluorescence: PDK2 Antibody [NBP1-87307]
Immunocytochemistry/Immunofluorescence: PDK2 Antibody [NBP1-87307] - Staining of human cell line A-431 shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: PDK2 Antibody [NBP1-87307]](https://resources.bio-techne.com/images/products/PDK2-Antibody-Immunohistochemistry-Paraffin-NBP1-87307-img0006.jpg "Immunohistochemistry-Paraffin: PDK2 Antibody [NBP1-87307]")
Immunohistochemistry-Paraffin: PDK2 Antibody [NBP1-87307]
Immunohistochemistry-Paraffin: PDK2 Antibody [NBP1-87307] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.Applications for PDK2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. ICC/IF reported in the literature (PMID: 25991547) and WB (PMID: 30457021).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PDK2
Long Name
[Pyruvate dehydrogenase
Alternate Names
PDH kinase 2, PDHK2, PDKII
Gene Symbol
PDK2
Additional PDK2 Products
Product Documents for PDK2 Antibody
Product Specific Notices for PDK2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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