PDK3 Antibody (2B11)
Novus Biologicals, part of Bio-Techne | Catalog # H00005165-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2B11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PDK3 (AAH15948, 174 a.a. ~ 263 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDR
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 22429297). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
PDK3 - pyruvate dehydrogenase kinase, isozyme 3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PDK3 Antibody (2B11)
![Western Blot: PDK3 Antibody (2B11) [H00005165-M01]](https://resources.bio-techne.com/images/products/PDK3-Antibody-2B11-Western-Blot-H00005165-M01-img0006.jpg "Western Blot: PDK3 Antibody (2B11) [H00005165-M01]")
Western Blot: PDK3 Antibody (2B11) [H00005165-M01]
Western Blot: PDK3 Antibody (2B11) [H00005165-M01] - Analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.![Western Blot: PDK3 Antibody (2B11) [H00005165-M01]](https://resources.bio-techne.com/images/products/PDK3-Antibody-2B11-Western-Blot-H00005165-M01-img0005.jpg "Western Blot: PDK3 Antibody (2B11) [H00005165-M01]")
Western Blot: PDK3 Antibody (2B11) [H00005165-M01]
Western Blot: PDK3 Antibody (2B11) [H00005165-M01] - PDK3 monoclonal antibody (M01), clone 2B11 Analysis of PDK3 expression in MCF-7.![ELISA: PDK3 Antibody (2B11) [H00005165-M01]](https://resources.bio-techne.com/images/products/PDK3-Antibody-2B11-ELISA-H00005165-M01-img0007.jpg "ELISA: PDK3 Antibody (2B11) [H00005165-M01]")
ELISA: PDK3 Antibody (2B11) [H00005165-M01]
ELISA: PDK3 Antibody (2B11) [H00005165-M01] - Detection limit for recombinant GST tagged PDK3 is approximately 0.03ng/ml as a capture antibody.Applications for PDK3 Antibody (2B11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PDK3
Long Name
[Pyruvate dehydrogenase
Alternate Names
PDHK3
Entrez Gene IDs
5165 (Human)
Gene Symbol
PDK3
OMIM
602526 (Human)
UniProt
Additional PDK3 Products
Product Documents for PDK3 Antibody (2B11)
Product Specific Notices for PDK3 Antibody (2B11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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