PDK3 Antibody (3A1)
Novus Biologicals, part of Bio-Techne | Catalog # H00005165-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3A1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PDK3 (AAH15948, 174 a.a. ~ 263 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDR
Specificity
PDK3 (3A1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PDK3 Antibody (3A1)
![Western Blot: PDK3 Antibody (3A1) [H00005165-M02]](https://resources.bio-techne.com/images/products/PDK3-Antibody-3A1-Western-Blot-H00005165-M02-img0004.jpg "Western Blot: PDK3 Antibody (3A1) [H00005165-M02]")
Western Blot: PDK3 Antibody (3A1) [H00005165-M02]
Western Blot: PDK3 Antibody (3A1) [H00005165-M02] - PDK3 monoclonal antibody (M02), clone 3A1 Analysis of PDK3 expression in A-431.
Sandwich ELISA: PDK3 Antibody (3A1) [H00005165-M02] - Detection limit for recombinant GST tagged PDK3 is approximately 0.03ng/ml as a capture antibody.
Applications for PDK3 Antibody (3A1)
Application
Recommended Usage
Sandwich ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using H00005165-M02 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PDK3
Long Name
[Pyruvate dehydrogenase
Alternate Names
PDHK3
Entrez Gene IDs
5165 (Human)
Gene Symbol
PDK3
OMIM
602526 (Human)
UniProt
Additional PDK3 Products
Product Documents for PDK3 Antibody (3A1)
Product Specific Notices for PDK3 Antibody (3A1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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