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PDK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13745

Novus Biologicals, part of Bio-Techne
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NBP2-13745
NBP2-13745-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PDK3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NKSPEDPQVLDNFLQVLIKVRNRHNDV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDK3 Antibody

Western Blot: PDK3 Antibody [NBP2-13745]

Western Blot: PDK3 Antibody [NBP2-13745]

Western Blot: PDK3 Antibody [NBP2-13745] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PDK3 Antibody [NBP2-13745]

Immunohistochemistry-Paraffin: PDK3 Antibody [NBP2-13745]

Immunohistochemistry-Paraffin: PDK3 Antibody [NBP2-13745] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

Applications for PDK3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDK3

PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Long Name

[Pyruvate dehydrogenase

Alternate Names

PDHK3

Gene Symbol

PDK3

Additional PDK3 Products

Product Documents for PDK3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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