PDK4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-07049

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.24 mg/ml

View all PDK4 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 200-250). [Swiss-Prot# Q16654]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (94%) and mouse (88%).

Localization

Mitochondrion matrix.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDK4 Antibody - BSA Free

Western Blot: PDK4 AntibodyBSA Free [NBP1-07049]

Western Blot: PDK4 AntibodyBSA Free [NBP1-07049]

Western Blot: PDK4 Antibody [NBP1-07049] - Western Blot on human heart protein lysate.
Immunocytochemistry/ Immunofluorescence: PDK4 Antibody - BSA Free [NBP1-07049]

Immunocytochemistry/ Immunofluorescence: PDK4 Antibody - BSA Free [NBP1-07049]

Immunocytochemistry/Immunofluorescence: PDK4 Antibody [NBP1-07049] - PDK4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for PDK4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is seen at ~49 kDa. In ICC/IF mitochondrial staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.24 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDK4

PDK4 (pyruvate dehydrogenase kinase isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptional initiation complex responsible for PDK4 activation. Insulin promotes inactivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.

Alternate Names

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial, EC 2.7.11, EC 2.7.11.2, FLJ40832, Pyruvate dehydrogenase kinase isoform 4, pyruvate dehydrogenase kinase, isoenzyme 4, pyruvate dehydrogenase kinase, isozyme 4, pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Entrez Gene IDs

5166 (Human); 89813 (Rat)

Gene Symbol

PDK4

UniProt

Q16654 (Human)

Additional PDK4 Products

Product Documents for PDK4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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