PDK4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54723

Novus Biologicals, part of Bio-Techne
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NBP1-54723
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all PDK4 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to PDK4(pyruvate dehydrogenase kinase, isozyme 4) The peptide sequence was selected from the N terminal of PDK4. Peptide sequence MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACE. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PDK4 Antibody

Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723] - Titration: 0.2-1 ug/ml Positive Control: Transfected 293T.
Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723]

Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723]

Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.
Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723] - Sample Type: Huh7 HepG2 (50ug) Primary Antibody Dilution: 1:500 Image Submitted By: Partha Kasturi University of Kansas Medical Center.

Applications for PDK4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDK4

PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

Alternate Names

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial, EC 2.7.11, EC 2.7.11.2, FLJ40832, Pyruvate dehydrogenase kinase isoform 4, pyruvate dehydrogenase kinase, isoenzyme 4, pyruvate dehydrogenase kinase, isozyme 4, pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Gene Symbol

PDK4

UniProt

Q16654 (Human)

Additional PDK4 Products

Product Documents for PDK4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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