PDLIM1 Antibody (5X10L2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15689
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5X10L2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PDLIM1 (O00151). LQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFF
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDLIM1 Antibody (5X10L2)
![Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]](https://resources.bio-techne.com/images/products/PDLIM1-Antibody-5X10L2-Western-Blot-NBP3-15689-img0002.jpg "Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]")
Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]
Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689] - Western blot analysis of extracts of various cell lines, using PDLIM1 antibody (NBP3-15689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunocytochemistry/ Immunofluorescence: PDLIM1 Antibody (5X10L2) [NBP3-15689]](https://resources.bio-techne.com/images/products/PDLIM1-Antibody-5X10L2-Immunocytochemistry-Immunofluorescence-NBP3-15689-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PDLIM1 Antibody (5X10L2) [NBP3-15689]")
Immunocytochemistry/ Immunofluorescence: PDLIM1 Antibody (5X10L2) [NBP3-15689]
Immunocytochemistry/Immunofluorescence: PDLIM1 Antibody (5X10L2) [NBP3-15689] - Immunofluorescence analysis of NIH/3T3 cells using PDLIM1 Rabbit mAb (NBP3-15689) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]](https://resources.bio-techne.com/images/products/PDLIM1-Antibody-5X10L2-Western-Blot-NBP3-15689-img0003.jpg "Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]")
Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]
Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689] - Western blot analysis of extracts of Mouse thymus, using PDLIM1 antibody (NBP3-15689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Applications for PDLIM1 Antibody (5X10L2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PDLIM1
Alternate Names
CLIM1carboxyl terminal LIM domain protein 1, CLP-36, CLP36carboxy terminal LIM domain protein 1, C-terminal LIM domain protein 1, elfin, enigma, hCLIM1, LIM domain protein CLP-36, PDZ and LIM domain 1, PDZ and LIM domain 1 (elfin), PDZ and LIM domain protein 1
Gene Symbol
PDLIM1
Additional PDLIM1 Products
Product Documents for PDLIM1 Antibody (5X10L2)
Product Specific Notices for PDLIM1 Antibody (5X10L2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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