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PDLIM5 Antibody (3E11-F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00010611-M01

Novus Biologicals, part of Bio-Techne
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H00010611-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E11-F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PDLIM5 (AAH08741, 1 a.a. ~ 596 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKAAQANVRIGDVVLSIDGINAQGMTHLEAQNKIKGCTGSLNMTLQRASAAPKPEPVPVQKGEPKEVVKPVPITSPAVSKVTSTNNMAYNKAPRPFGSVSSPKVTSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKKRLIEDTEDWRPRTGTTQSRSFRILAQITGTEHLKESEADNTKKANNSQEPSPQLASSVASTRSMPESLDSPTSGRPGVTSLTTAAAFKPVGSTGVIKSPSWQRPNQGVPSTGRISNSAAYSGSVAPANSALGQTQPSDQDTLVQRAEHIPAGKRTPMCAHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYEKFFAPECGRCQRKILGEVINALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICHGCEFPIEAGDMFLEALGYTWHDTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF

Reactivity Notes

Human. Other species not tested.

Specificity

PDLIM5 - PDZ and LIM domain 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PDLIM5 Antibody (3E11-F6)

Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01]

Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01]

Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - PDLIM5 monoclonal antibody (M01), clone 3E11-F6. Analysis of PDLIM5 expression in HepG2.
Immunocytochemistry/ Immunofluorescence: PDLIM5 Antibody (3E11-F6) [H00010611-M01]

Immunocytochemistry/ Immunofluorescence: PDLIM5 Antibody (3E11-F6) [H00010611-M01]

Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Analysis of monoclonal antibody to PDLIM5 on A-431 cell. Antibody concentration 10 ug/ml.
Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01]

Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01]

Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Western Blot analysis of PDLIM5 expression in A-431 ( Cat # L015V1 ).

Applications for PDLIM5 Antibody (3E11-F6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDLIM5

The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.

Alternate Names

Enh, ENH1, ENHLIM, Enigma homolog, enigma-like LIM domain protein, Enigma-like PDZ and LIM domains protein, PDZ and LIM domain 5, PDZ and LIM domain protein 5

Entrez Gene IDs

10611 (Human)

Gene Symbol

PDLIM5

OMIM

605904 (Human)

Additional PDLIM5 Products

Product Documents for PDLIM5 Antibody (3E11-F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDLIM5 Antibody (3E11-F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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