PDLIM5 Antibody (OTI1H10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73317

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1H10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PDLIM5 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDLIM5(NP_006448) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

63.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDLIM5 Antibody (OTI1H10) - Azide and BSA Free

Western Blot: PDLIM5 Antibody (OTI1H10)Azide and BSA Free [NBP2-73317]

Western Blot: PDLIM5 Antibody (OTI1H10)Azide and BSA Free [NBP2-73317]

Western Blot: PDLIM5 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73317] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.
Flow Cytometry: PDLIM5 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73317]

Flow Cytometry: PDLIM5 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73317]

Flow Cytometry: PDLIM5 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73317] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PDLIM5 antibody, and then analyzed by flow cytometry.

Applications for PDLIM5 Antibody (OTI1H10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDLIM5

PDLIM5 is encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. [provided by RefSeq]

Alternate Names

Enh, ENH1, ENHLIM, Enigma homolog, enigma-like LIM domain protein, Enigma-like PDZ and LIM domains protein, PDZ and LIM domain 5, PDZ and LIM domain protein 5

Gene Symbol

PDLIM5

Additional PDLIM5 Products

Product Documents for PDLIM5 Antibody (OTI1H10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDLIM5 Antibody (OTI1H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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