PDLIM5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84842

Novus Biologicals, part of Bio-Techne
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NBP1-84842
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDLIM5 Antibodies »

Product Summary for PDLIM5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TTQSRSFRILAQITGTEHLKESEADNTKKANNSQEPSPQLASSVASTRSMPESLDSPTSGRPGVTSLTTAAAFKPVGSTGVIKSPSWQRPNQGVPSTGRISNSATYSGSVAPANSALGQTQPSDQDTLVQRAEHIPAGKRTPMC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDLIM5 Antibody

Western Blot: PDLIM5 Antibody [NBP1-84842]

Western Blot: PDLIM5 Antibody [NBP1-84842]

Western Blot: PDLIM5 Antibody [NBP1-84842] - Analysis in human cell line A-549.
Immunocytochemistry/ Immunofluorescence: PDLIM5 Antibody [NBP1-84842]

Immunocytochemistry/ Immunofluorescence: PDLIM5 Antibody [NBP1-84842]

Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84842] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84842]

Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84842]

Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84842] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Applications for PDLIM5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:10-1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDLIM5

PDLIM5 is encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. [provided by RefSeq]

Alternate Names

Enh, ENH1, ENHLIM, Enigma homolog, enigma-like LIM domain protein, Enigma-like PDZ and LIM domains protein, PDZ and LIM domain 5, PDZ and LIM domain protein 5

Gene Symbol

PDLIM5

Additional PDLIM5 Products

Product Documents for PDLIM5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDLIM5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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