PELO Antibody (2C2)
Novus Biologicals, part of Bio-Techne | Catalog # H00053918-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2C2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PELO (NP_057030.3, 291 a.a. ~ 385 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEED
Specificity
Reacts with pelota homolog (Drosophila).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for PELO Antibody (2C2)
![Western Blot: PELO Antibody (2C2) [H00053918-M03]](https://resources.bio-techne.com/images/products/PELO-Antibody-2C2-Western-Blot-H00053918-M03-img0003.jpg "Western Blot: PELO Antibody (2C2) [H00053918-M03]")
Western Blot: PELO Antibody (2C2) [H00053918-M03]
Western Blot: PELO Antibody (2C2) [H00053918-M03] - Analysis of PELO expression in transfected 293T cell line by PELO monoclonal antibody (M03), clone 2C2. Lane 1: PELO transfected lysate (Predicted MW: 43.4 KDa). Lane 2: Non-transfected lysate.![Immunoprecipitation: PELO Antibody (2C2) [H00053918-M03]](https://resources.bio-techne.com/images/products/PELO-Antibody-2C2-Immunoprecipitation-H00053918-M03-img0001.jpg "Immunoprecipitation: PELO Antibody (2C2) [H00053918-M03]")
Immunoprecipitation: PELO Antibody (2C2) [H00053918-M03]
Immunoprecipitation: PELO Antibody (2C2) [H00053918-M03] - Analysis of PELO transfected lysate using anti-PELO monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PELO monoclonal antibody.
Sandwich ELISA: PELO Antibody (2C2) [H00053918-M03] - Detection limit for recombinant GST tagged PELO is 0.03 ng/ml as a capture antibody.
Applications for PELO Antibody (2C2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PELO
Alternate Names
EC 3.1, pelota (Drosophila) homolog, pelota homolog (Drosophila), PRO1770, protein pelota homolog
Entrez Gene IDs
53918 (Human)
Gene Symbol
PELO
UniProt
Additional PELO Products
Product Documents for PELO Antibody (2C2)
Product Specific Notices for PELO Antibody (2C2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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