PELO Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19769
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Predicted:
Bovine (98%), Chicken (95%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PELO. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (82%), Xenopus laevis (83%).
Localization
Nucleus (Potential); Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PELO Antibody
Western Blot: PELO Antibody [NBP2-19769]
Western Blot: PELO Antibody [NBP2-19769] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: PELO Antibody [NBP2-19769]
Immunocytochemistry/Immunofluorescence: PELO Antibody [NBP2-19769] - Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PELO protein stained by PELO antibody diluted at 1:500. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: PELO Antibody [NBP2-19769]
Immunohistochemistry-Paraffin: PELO Antibody [NBP2-19769] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.Applications for PELO Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PELO
Alternate Names
EC 3.1, pelota (Drosophila) homolog, pelota homolog (Drosophila), PRO1770, protein pelota homolog
Gene Symbol
PELO
UniProt
Additional PELO Products
Product Documents for PELO Antibody
Product Specific Notices for PELO Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.