PELP1 Antibody (6H4)

Western Blot: PELP1 Antibody (6H4) [NBP3-15157]

Western Blot: PELP1 Antibody (6H4) [NBP3-15157] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate. All lanes: PELP1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 KDa. Observed band size: 160 KDa.

Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157]

Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157] - IHC image of PELP1 antibody diluted at 1:100 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157]

Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157] - IHC image of PELP1 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.