PELP1 Antibody (JE02-42)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32734
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE02-42
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide. (Uniprot: Q8IZL8)
Localization
Nucleus, nucleolus, nucleoplasm, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PELP1 Antibody (JE02-42)
Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734]
Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.Lane 1: HeLa cell lysate (input)
Lane 2: NBP3-32734 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL
Western Blot: PELP1 Antibody (JE02-42) [NBP3-32734]
Western Blot: PELP1 Antibody (JE02-42) [NBP3-32734] - Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution.Lane 1: SW620 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: NIH/3T3 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 120 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: PELP1 Antibody (JE02-42) [NBP3-32734]
Immunohistochemistry: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32734) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for PELP1 Antibody (JE02-42)
Application
Recommended Usage
Flow Cytometry
1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Paraffin
1:1000
Immunoprecipitation
1-2ug/sample
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PELP1
Alternate Names
HMX3, MNARmodulator of nongenomic activity of estrogen receptor, Modulator of non-genomic activity of estrogen receptor, P160, proline and glutamic acid rich nuclear protein, proline, glutamate and leucine rich protein 1, proline, glutamic acid and leucine rich protein 1, proline-, glutamic acid- and leucine-rich protein 1, proline-, glutamic acid-, leucine-rich protein 1, Transcription factor HMX3
Gene Symbol
PELP1
Additional PELP1 Products
Product Documents for PELP1 Antibody (JE02-42)
Product Specific Notices for PELP1 Antibody (JE02-42)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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