PEPD Antibody (1D5-H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00005184-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D5-H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PEPD Antibodies »

Product Specifications

Immunogen

PEPD (AAH15027, 1 a.a. ~ 493 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVAEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQEPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Specificity

PEPD - peptidase D

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PEPD Antibody (1D5-H3)

Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01]

Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01]

Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.Lane 1: PEPD transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PEPD Antibody (1D5-H3) [H00005184-M01]

Immunocytochemistry/ Immunofluorescence: PEPD Antibody (1D5-H3) [H00005184-M01]

Immunocytochemistry/Immunofluorescence: PEPD Antibody (1D5-H3) [H00005184-M01] - Analysis of monoclonal antibody to PEPD on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01]

Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01]

Western Blot: PEPD Antibody (1D5-H3) [H00005184-M01] - PEPD monoclonal antibody (M01), clone 1D5-H3 Analysis of PEPD expression in HepG2.
View 2 more images

Applications for PEPD Antibody (1D5-H3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PEPD

Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of iminoacids.

Alternate Names

aminoacyl-L-proline hydrolase, EC 3.4.13.9, Imidodipeptidase, MGC10905, peptidase Dimidodipeptidase, PRD, Prolidase, Proline dipeptidase, xaa-Pro dipeptidase, X-Pro dipeptidase

Entrez Gene IDs

5184 (Human)

Gene Symbol

PEPD

OMIM

170100 (Human)

UniProt

AAH15027 (Human)

Additional PEPD Products

Product Documents for PEPD Antibody (1D5-H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PEPD Antibody (1D5-H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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