Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PEPP2 Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 876 to 925 of human Pleckstrin Homology Domain Containing, Family A Member 6 using the numbering given in entry NP_061885.3 (GeneID 54477).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEPP2 Antibody

Western Blot: PEPP2 Antibody [NBP2-37696]

Western Blot: PEPP2 Antibody [NBP2-37696]

Western Blot: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PLEKHA5 antibody used for IP at 6 ug per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody. For blotting immunoprecipitated PLEKHA5, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696]

Immunoprecipitation: PEPP2 Antibody [NBP2-37696] - Detection of human PLEKHA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLEKHA5 antibody NBP2-37696 used for IP at 6 ug per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody NBP2-37695. For blotting immunoprecipitated PLEKHA5, NBP2-37696 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PEPP2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PEPP2

PEPP2 is a member of the homeobox PEPP subfamily. PEPP2 is highly expressed in heart and kidney, and also in reproductive tissues; the human homolog is specifically expressed in testis.

Alternate Names

FLJ10667, FLJ26734, KIAA1686FLJ31492, PEPP-2, PEPP2phosphoinositol 3-phosphate-binding protein-2, PH domain-containing family A member 5, Phosphoinositol 3-phosphate-binding protein 2, pleckstrin homology domain containing, family A member 5, pleckstrin homology domain-containing family A member 5

Gene Symbol

PLEKHA5

UniProt

NP_061885.3 (Human)

Additional PEPP2 Products

Product Documents for PEPP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PEPP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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