Pepsinogen I Antibody (HL2137) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25594
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2137
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Pepsinogen I Antibody (HL2137) - Azide and BSA Free
Immunogen
Recombinant fragmemt of human Pepsinogen I
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Pepsinogen I Antibody (HL2137) - Azide and BSA Free
ELISA: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] -
ELISA: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Indirect ELISA analysis was performed by coating the plate with recombinant NS0 cells expressed, full-length human pepsinogen A protein (97.56-1.52 nM). Coated protein was probed with Pepsinogen I antibody [HL2137] (NBP3-25594) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
Western Blot: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] -
Western Blot: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Various tissue extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] -
Immunohistochemistry-Paraffin: Pepsinogen I Antibody (HL2137) - Azide and BSA Free [NBP3-25594] - Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human stomach. Pepsinogen I stained by Pepsinogen I antibody [HL2137] (NBP3-25594) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Pepsinogen I Antibody (HL2137) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pepsinogen I
Alternate Names
pepsinogen 3, group I (pepsinogen A)
Gene Symbol
PGA3
Additional Pepsinogen I Products
Product Documents for Pepsinogen I Antibody (HL2137) - Azide and BSA Free
Product Specific Notices for Pepsinogen I Antibody (HL2137) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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