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Name: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody
Brand: Novus Biologicals
SKU: NBP2-34075

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Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34075

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Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PAM Antibodies »

Product Summary for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIPVKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -

Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Analysis in human heart muscle and liver tissues using NBP2-34075 antibody. Corresponding PAM RNA-seq data are presented for the same tissues.

Western Blot: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -

Western Blot: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Analysis in human cell line A-431 and human cell line MCF-7.

Immunocytochemistry/ Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]

Immunocytochemistry/Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

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Applications for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAM

PAM encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq]

Long Name

Peptidylglycine Alpha-Amidating Monooxygenase

Alternate Names

PAL, PHM

Gene Symbol

PAM

UniProt

P19021

Additional PAM Products

Product Documents for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34075

Key Product Details

Validated by

Species Reactivity

Validated:

Predicted:

Applications

Label

Antibody Source

Concentration

View all PAM Antibodies »

Product Summary for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Scientific Data Images for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -

Western Blot: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -

Immunocytochemistry/ Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]

Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]

Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -

Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -

Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -

Applications for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: PAM

Long Name

Alternate Names

Gene Symbol

UniProt

Additional PAM Products

Product Documents for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody