Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34075
![Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]](https://resources.bio-techne.com/images/products/Peptidylglycine-alpha-Amidating-Monooxygenase-PAM-Antibody-Immunohistochemistry-Paraffin-NBP2-34075-img0010.jpg "Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIPVKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody
Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] -
Immunohistochemistry-Paraffin: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Analysis in human heart muscle and liver tissues using NBP2-34075 antibody. Corresponding PAM RNA-seq data are presented for the same tissues.![Immunocytochemistry/ Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]](https://resources.bio-techne.com/images/products/Peptidylglycine-alpha-Amidating-Monooxygenase-PAM-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34075-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]")
Immunocytochemistry/ Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075]
Immunocytochemistry/Immunofluorescence: Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [NBP2-34075] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Applications for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PAM
Long Name
Peptidylglycine Alpha-Amidating Monooxygenase
Alternate Names
PAL, PHM
Gene Symbol
PAM
UniProt
Additional PAM Products
Product Documents for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody
Product Specific Notices for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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