PER2 Antibody (2K9Y0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16557
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: PER2 Antibody (2K9Y0) [NBP3-16557]](https://resources.bio-techne.com/images/products/PER2-Antibody-2K9Y0-Immunocytochemistry-Immunofluorescence-NBP3-16557-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PER2 Antibody (2K9Y0) [NBP3-16557]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2K9Y0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PER2 Antibody (2K9Y0)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PER2 (O15055). MNGYAEFPPSPSNPTKEPVEPQPSQVPLQEDVDMSSGSSGHETNENCSTGRDSQGSDCDDSGKELGMLVEPPDARQSPDTFSLMMAKSEHNPSTSGCSSD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PER2 Antibody (2K9Y0)
![Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557]](https://resources.bio-techne.com/images/products/PER2-Antibody-2K9Y0-Western-Blot-NBP3-16557-img0003.jpg "Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557]")
Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557]
Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557] - Western blot analysis of extracts of various cell lines, using PER2 Rabbit mAb (NBP3-16557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.![Immunocytochemistry/ Immunofluorescence: PER2 Antibody (2K9Y0) [NBP3-16557]](https://resources.bio-techne.com/images/products/PER2-Antibody-2K9Y0-Immunocytochemistry-Immunofluorescence-NBP3-16557-img0002.jpg "Immunocytochemistry/ Immunofluorescence: PER2 Antibody (2K9Y0) [NBP3-16557]")
Immunocytochemistry/ Immunofluorescence: PER2 Antibody (2K9Y0) [NBP3-16557]
Immunocytochemistry/Immunofluorescence: PER2 Antibody (2K9Y0) [NBP3-16557] - Immunofluorescence analysis of NIH-3T3 cells using PER2 Rabbit mAb (NBP3-16557) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: PER2 Antibody (2K9Y0) [NBP3-16557] - Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using PER2 antibody (NBP3-16557) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Applications for PER2 Antibody (2K9Y0)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:50 - 1:200
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PER2
Alternate Names
Circadian clock protein PERIOD 2, FASPS, hPER2, KIAA0347period 2, period (Drosophila) homolog 2, period circadian protein 2, period circadian protein homolog 2, period homolog 2 (Drosophila)
Gene Symbol
PER2
Additional PER2 Products
Product Documents for PER2 Antibody (2K9Y0)
Product Specific Notices for PER2 Antibody (2K9Y0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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