PER2 Antibody (JE33-79)

Western Blot: PER2 Antibody (JE33-79) [NBP3-32735]

Western Blot: PER2 Antibody (JE33-79) [NBP3-32735] - Western blot analysis of PER2 on K562 cell lysates with Rabbit anti-PER2 antibody (NBP3-32735) at 1/500 dilution.

Lysates/proteins at 10 ug/Lane.

Predicted band size: 137 kDa
Observed band size: 180 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32735) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PER2 Antibody (JE33-79) [NBP3-32735]

Immunohistochemistry: PER2 Antibody (JE33-79) [NBP3-32735] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PER2 antibody (NBP3-32735) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32735) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.