PER2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-125

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PER2 Antibodies »

Product Specifications

Immunogen

A partial synthetic peptide made to an internal portion of the human PER2 protein (between amino acids 100-150) [UniProt# O15055].

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30016666). Use in Rat reported in scientific literature (PMID:32119846).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PER2 Antibody - BSA Free

Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.
Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/ Immunofluorescence: PER2 Antibody - BSA Free [NB100-125]

Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 AntibodyBSA Free [NB100-125]

Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.
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Applications for PER2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200. Use reported in scientific literature (PMID 31822134)

Immunoprecipitation

reported in scientific literature (PMID 31390562)

Western Blot

1 - 2 ug/ml
Application Notes
This PER2 antibody is useful in Western blot and Immunocytochemistry/Immunofluorescence. It has been tested against expressed fragment of human PER2 (bacterially expressed fragment from BL21 cells), or full-length protein expressed in in vitro translation using rabbit reticulocyte lysate. In Western blot, a band can be seen at ~200 kDa representing PER2.

Reviewed Applications

Read 3 reviews rated 3.7 using NB100-125 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PER2

PER2 (period circadian protein homolog 2 or circadian clock protein PERIOD 2) is a component of the circadian clock mechanism which is essential for generating circadian rhythms and PER2 acts as a negative element in the circadian transcriptional loop. PER2 modulates the clock function by interacting with other circadian regulatory proteins for transporting them to nucleus, and negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. PER2 forms a component of the circadian core oscillator, which also includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS proteinsetc. PER2 interacts with NFIL3, PER1, PER3, CRY1 and CRY2 proteins, and its interaction with CSNK1D or CSNK1E promotes nuclear localization of PER proteins. PER2 is a nuclear protein and its nucleo-cytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. CSNK1E and CSNK1D facilitate PER2 phosphorylation that follows its degradation. PER2 defects have been found to be responsible for familial advanced sleep-phase syndrome (FASPS).

Alternate Names

Circadian clock protein PERIOD 2, FASPS, hPER2, KIAA0347period 2, period (Drosophila) homolog 2, period circadian protein 2, period circadian protein homolog 2, period homolog 2 (Drosophila)

Entrez Gene IDs

8864 (Human)

Gene Symbol

PER2

UniProt

O15055 (Human)

Additional PER2 Products

Product Documents for PER2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PER2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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