Perilipin-3/TIP47 Antibody [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40764R

Novus Biologicals, part of Bio-Techne
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NB110-40764R
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Perilipin-3 Antibodies »

Product Summary for Perilipin-3/TIP47 Antibody [DyLight 550]

Immunogen

A synthetic peptide made to a region within the C-terminus (within residues 350-435) of the human TIP47 protein. [Swiss-Prot# O60664]

Localization

Cytoplasm. Endosome. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Perilipin-3/TIP47 Antibody [DyLight 550]

Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [DyLight 550] [NB110-40764R] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Perilipin-3/TIP47 Antibody conjugated to DyLight 550 (NB110-40764R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Perilipin-3/TIP47 Antibody [DyLight 550]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Perilipin-3

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. TIP47 has been localized in milk fat globule membranes of human and bovine origin. It has been described as a placental protein, as well. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, this protein is probably significant as an oncodevelopmental marker.

Alternate Names

M6PRBP1, Perilipin3, PLIN3, PP17, TIP47

Gene Symbol

PLIN3

Additional Perilipin-3 Products

Product Documents for Perilipin-3/TIP47 Antibody [DyLight 550]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Perilipin-3/TIP47 Antibody [DyLight 550]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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