Perilipin-3/TIP47 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40765

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Perilipin-3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a region within the N-terminus (within residues 1-100) of the mouse TIP47 protein. [Swiss-Prot# Q9DBG5]

Localization

Cytoplasm. Endosome. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Perilipin-3/TIP47 Antibody

Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765]

Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765]

Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.
Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765]

Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765]

Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).
Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765]

Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765]

Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765] - Simple Western lane view shows a specific band for TIP47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Perilipin-3/TIP47 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Simple Western

1:100

Western Blot

1:1000-1:5000
Application Notes
This TIP47 antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western Blot analysis, a band is seen approx. 47 kDa (isoform B, containing the first 184 residues that isoform A is lacking). In ICC/IF, endosomal staining was observed in U2OS cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Perilipin-3

TIP47 (tail-interacting protein 47) is a member of PAT family of proteins (perilipin, ADRP, S3-12 and OXPAT) which plays a key role in the packaging/storage of neutral lipids and is required for mannose 6-phosphate receptors (M6PR) transport from endosomes to trans-Golgi network (TGN). TIP47 localizes in cytoplasm, endosome membrane as peripheral membrane protein towards cytoplasmic side, core of lipid bodies/ lipid droplet, in lipid sails and in the envelope, and it interacts with M6PR, IGF2R etc. via the cytoplasmic domain. As a cargo protein, TIP47 facilitates the delivery of M6PR from endosomes to TGN where it binds to cytoplasmic domains of cation-independent/-dependent M6PR and translocates to late endosomes by binding to Rab9 GTPase. Like other PAT family members, TIP47 associates with lipid droplets and influence insulin signaling as evidenced by combined knockout of ADRP /TIP47 leading to decreased insulin sensitivity. During HIV-1 infection, TIP47 acts as a cellular cofactor playing a part in Env incorporation, allowing the encounter and physical association between HIV-1 Gag and Env proteins during viral assembly process. TIP47 is identical to pregnancy-related placental tissue protein 17b (PP17b) and is overexpressed in cervical carcinoma patients wherein it is considered a serum tumor marker.

Alternate Names

M6PRBP1, Perilipin3, PLIN3, PP17, TIP47

Gene Symbol

PLIN3

Additional Perilipin-3 Products

Product Documents for Perilipin-3/TIP47 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Perilipin-3/TIP47 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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