Perilipin-3/TIP47 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06049

Novus Biologicals, part of Bio-Techne
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NBP1-06049
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Perilipin-3 Antibodies »

Product Specifications

Immunogen

This Perilipin-3/TIP47 Antibody was developed against a peptide with sequence C-NQKTPQDAEKDPAK corresponding to internal region according to Uniprot Mouse NP_080112.1.

Epitope

C-NQKTPQDAEKDPAK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Perilipin-3/TIP47 Antibody

Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-06049]

Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-06049]

Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-06049] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and lipid droplet staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for Perilipin-3/TIP47 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Perilipin-3

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. PLIN3 has been localized in milk fat globule membranes of human and bovine origin. It has also been described as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, TIP47 is probably significant as an oncodevelopmental marker.

Alternate Names

M6PRBP1, Perilipin3, PLIN3, PP17, TIP47

Entrez Gene IDs

66905 (Mouse); 316130 (Rat)

Gene Symbol

PLIN3

UniProt

NP_080112.1 (Mouse)

Additional Perilipin-3 Products

Product Documents for Perilipin-3/TIP47 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Perilipin-3/TIP47 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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