Peroxiredoxin 3 Antibody (AT1F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12869

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # AT1F8

Format

BSA Free

Concentration

1 mg/ml

View all Peroxiredoxin 3 Antibodies »

Product Specifications

Immunogen

Recombinant human Peroxiredoxin3 (63-256aa) purified from E. coli (Accession number: NP_006784)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Peroxiredoxin 3 Antibody (AT1F8) - BSA Free

Western Blot: Peroxiredoxin 3 Antibody (AT1F8)BSA Free [NBP3-12869]

Western Blot: Peroxiredoxin 3 Antibody (AT1F8)BSA Free [NBP3-12869]

Western Blot: Peroxiredoxin 3 Antibody (AT1F8) [NBP3-12869] - The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-12869 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: Recombinant human Peroxiredoxin 3 protein. Lane 2: HeLa cell lysate. Lane 3: HepG2 cell lysate. Lane 4: TF1 cell lysate. Lane 5: U87MG cell lysate. Lane 6: Raji cell lysate. Lane 7: 293T cell lysate. Lane 8: Jurkat cell lysate. Lane 9: MCF7 cell lysate
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody (AT1F8) - BSA Free [NBP3-12869]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody (AT1F8) - BSA Free [NBP3-12869]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (AT1F8) [NBP3-12869] - HeLa cells were stained with NBP3-12869 (1:100). Alexa Fluor 488-conjugated secondary antibody was used (green). DAPI was stained the cell nucleus (blue).

Applications for Peroxiredoxin 3 Antibody (AT1F8) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 3

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.

Alternate Names

AOP-1, MER5, PRDX3, SP-22

Gene Symbol

PRDX3

Additional Peroxiredoxin 3 Products

Product Documents for Peroxiredoxin 3 Antibody (AT1F8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 3 Antibody (AT1F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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