Peroxiredoxin 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19777

Novus Biologicals, part of Bio-Techne
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NBP2-19777
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (91%), Porcine (90%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Peroxiredoxin 3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 3. The exact sequence is proprietary.

Reactivity Notes

Rat (85%), Bovine (89%), Xenopus laevis (82%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Peroxiredoxin 3 Antibody

Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777]

Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777]

Western Blot: Peroxiredoxin 3 Antibody [NBP2-19777] - Wild-type (WT) and Peroxiredoxin 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Peroxiredoxin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody [NBP2-19777]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 3 Antibody [NBP2-19777]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody [NBP2-19777] - HeLa cells. Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3]. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Peroxiredoxin 3 Antibody [NBP2-19777]

Immunohistochemistry-Paraffin: Peroxiredoxin 3 Antibody [NBP2-19777]

Immunohistochemistry-Paraffin: Peroxiredoxin 3 Antibody [NBP2-19777] - Immunohistochemical analysis of paraffin-embedded Lung AdC, using antibody at 1:100 dilution.
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Applications for Peroxiredoxin 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 3

This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

AOP-1, MER5, PRDX3, SP-22

Gene Symbol

PRDX3

UniProt

P30048

Additional Peroxiredoxin 3 Products

Product Documents for Peroxiredoxin 3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Peroxiredoxin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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