PET112L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80925

Novus Biologicals, part of Bio-Techne
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NBP1-80925
NBP1-80925-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PET112L Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WKREGKTPGQIVSEKQLELMQDQGALEQLCHSVMEAHPQVVMDVKNRNPRAINKLIGLVRKATQSRADPVMIKEILEKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PET112L Antibody

Western Blot: PET112L Antibody [NBP1-80925]

Western Blot: PET112L Antibody [NBP1-80925]

Western Blot: PET112L Antibody [NBP1-80925] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: PET112L Antibody [NBP1-80925]

Immunocytochemistry/ Immunofluorescence: PET112L Antibody [NBP1-80925]

Immunocytochemistry/Immunofluorescence: PET112L Antibody [NBP1-80925] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: PET112L Antibody [NBP1-80925]

Immunohistochemistry-Paraffin: PET112L Antibody [NBP1-80925]

Immunohistochemistry-Paraffin: PET112L Antibody [NBP1-80925] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Applications for PET112L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PET112L

Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (Potential)

Alternate Names

Cytochrome oxidase assembly factor PET112 homolog, EC 6.3.5, EC 6.3.5.-, Glu-ADT subunit B, mitochondrial, PET112 (yeast homolog)-like, PET112-like (yeast)

Entrez Gene IDs

5188 (Human)

Gene Symbol

PET112

UniProt

O75879 (Human)

Additional PET112L Products

Product Documents for PET112L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PET112L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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