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PFDN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86378

Novus Biologicals, part of Bio-Techne
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NBP1-86378
NBP1-86378-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAENSGRAGKSSGSGAGKGAVSAEQVIAGFNRLRQEQRGLASKAAELEMELNEHSLVIDTLKEVDETRKCYRMVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PFDN2 Antibody

Western Blot: PFDN2 Antibody [NBP1-86378]

Western Blot: PFDN2 Antibody [NBP1-86378]

Western Blot: PFDN2 Antibody [NBP1-86378] - Analysis in control (vector only transfected HEK293T lysate) and PFDN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PFDN2 Antibody [NBP1-86378]

Immunocytochemistry/ Immunofluorescence: PFDN2 Antibody [NBP1-86378]

Immunocytochemistry/Immunofluorescence: PFDN2 Antibody [NBP1-86378] - Staining of human cell line U-2 OS shows localization to cytosol.

Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.

Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.

Applications for PFDN2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PFDN2

The PFDN2 gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The

Alternate Names

PFD2, prefoldin 2, prefoldin subunit 2

Gene Symbol

PFDN2

Additional PFDN2 Products

Product Documents for PFDN2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PFDN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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