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PFDN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00279

Novus Biologicals, part of Bio-Techne
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NBP3-00279-50ul
NBP3-00279-100ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human PFDN2 (Uniprot#: Q9UHV9; Met1-Ser154)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PFDN2 Antibody

Western Blot: PFDN2 Antibody [NBP3-00279]

Western Blot: PFDN2 Antibody [NBP3-00279]

Western Blot: PFDN2 Antibody [NBP3-00279] - Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:17 kDa. Observed band size:17 kDa. (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: PFDN2 Antibody [NBP3-00279]

Immunoprecipitation: PFDN2 Antibody [NBP3-00279]

Immunoprecipitation: PFDN2 Antibody [NBP3-00279] - Lane A:0.5 mg Jurkat Whole Cell Lysate. 2 uL anti-PFDN2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PFDN2 rabbit polyclonal antibody,at 1:200 dilution . Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa.

Applications for PFDN2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PFDN2

The PFDN2 gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The

Alternate Names

PFD2, prefoldin 2, prefoldin subunit 2

Gene Symbol

PFDN2

Additional PFDN2 Products

Product Documents for PFDN2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PFDN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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