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PFDN5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82639

Novus Biologicals, part of Bio-Techne
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NBP1-82639

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: INITELNLPQLEMLKNQLDQEVEFLSTSIAQLKVVQTKYVEAKDCLNVLNKSNEGKELLVPLTSSMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTA

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26619345).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PFDN5 Antibody

Western Blot: PFDN5 Antibody [NBP1-82639]

Western Blot: PFDN5 Antibody [NBP1-82639]

Western Blot: PFDN5 Antibody [NBP1-82639] - Analysis in control (vector only transfected HEK293T lysate) and PFDN5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PFDN5 Antibody [NBP1-82639]

Immunohistochemistry-Paraffin: PFDN5 Antibody [NBP1-82639]

Immunohistochemistry-Paraffin: PFDN5 Antibody [NBP1-82639] - Staining of human small intestine shows strong cytoplasmic and membrane positivity in glandular cells.

Applications for PFDN5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB, ICC/IF reported in scientific literature (PMID: 26619345). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PFDN5

PFDN5 encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

c-myc binding protein, C-Myc-binding protein Mm-1, MM-1, MM1MGC71907, Myc modulator 1, myc modulator-1, PFD5MGC5329, prefoldin 5, prefoldin subunit 5

Gene Symbol

PFDN5

Additional PFDN5 Products

Product Documents for PFDN5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PFDN5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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