PFKFB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00005207-D01P

Novus Biologicals, part of Bio-Techne
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H00005207-D01P
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PFKFB1 Antibodies »

Product Specifications

Immunogen

PFKFB1 (NP_002616.1, 1 a.a. - 471 a.a.) full-length human protein. MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSRMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28706006).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for PFKFB1 Antibody

Western Blot: PFKFB1 Antibody [H00005207-D01P]

Western Blot: PFKFB1 Antibody [H00005207-D01P]

Western Blot: PFKFB1 Antibody [H00005207-D01P] - Analysis of PFKFB1 expression in transfected 293T cell line by PFKFB1 polyclonal antibody.Lane 1: PFKFB1 transfected lysate(54.70 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PFKFB1 Antibody [H00005207-D01P]

Immunocytochemistry/ Immunofluorescence: PFKFB1 Antibody [H00005207-D01P]

Immunocytochemistry/Immunofluorescence: PFKFB1 Antibody [H00005207-D01P] - Analysis of antibody to PFKFB1 on HeLa cell. Antibody concentration 20 ug/ml.

Applications for PFKFB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for WB and Functional.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PFKFB1

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2), and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B), and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Alternate Names

2-kinase:fructose-2,6-bisphosphatase, 6PF-2-K/Fru-2,6-P2ase liver isozyme, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1, F6PK, fructose-6-phosphate, MGC116715,6PF-2-K/Fru-2,6-P2ase 1, MGC116717, PFK/FBPase 1, PFRXHL2K

Gene Symbol

PFKFB1

UniProt

NP_002616.1 (Human)

Additional PFKFB1 Products

Product Documents for PFKFB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PFKFB1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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