PFKP Antibody (OTI1D6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01539

Novus Biologicals, part of Bio-Techne
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NBP2-01539
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-73344AF350
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Alexa Fluor 405
NBP2-73344AF405
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Alexa Fluor 488
NBP2-73344AF488
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Alexa Fluor 532
NBP2-73344AF532
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Alexa Fluor 594
NBP2-73344AF594
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Alexa Fluor 647
NBP2-73344AF647
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Alexa Fluor 700
NBP2-73344AF700
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Alexa Fluor 750
NBP2-73344AF750
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Allophycocyanin
NBP2-73344APC
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Allophycocyanin/Cy7
NBP2-73344APCCY7
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Biotin
NBP2-73344B
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CoraFluor 1
NBP2-73344CL1
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DyLight 350
NBP2-73344UV
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DyLight 405
NBP2-73344V
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DyLight 488
NBP2-73344G
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DyLight 550
NBP2-73344R
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DyLight 594
NBP2-73344DL594
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DyLight 650
NBP2-73344C
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DyLight 680
NBP2-73344FR
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DyLight 755
NBP2-73344IR
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FITC
NBP2-73344F
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HRP
NBP2-73344H
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Janelia Fluor 525
NBP2-73344JF525
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Janelia Fluor 549
NBP2-73344JF549
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Janelia Fluor 585
NBP2-73344JF585
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Janelia Fluor 635
NBP2-73344JF635
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Janelia Fluor 646
NBP2-73344JF646
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Janelia Fluor 669
NBP2-73344JF669
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mFluor Violet 450 SE
NBP2-73344MFV450
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mFluor Violet 500 SE
NBP2-73344MFV500
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mFluor Violet 610 SE
NBP2-73344MFV610
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PE
NBP2-73344PE
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PE/Atto594
NBP2-73344PEATT594
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PE/Cy5.5
NBP2-73344PECY55
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PE/Cy7
NBP2-73344PECY7
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PerCP
NBP2-73344PCP
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D6

Concentration

1 mg/ml

View all PFKP Antibodies »

Product Summary for PFKP Antibody (OTI1D6)

Immunogen

Full length human recombinant protein of human PFKP(NP_002618) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

85.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PFKP Antibody (OTI1D6)

Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539]

Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539]

Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cellsand PFKP-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-PFKP monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Immunocytochemistry/ Immunofluorescence: PFKP Antibody (OTI1D6) [NBP2-01539]

Immunocytochemistry/ Immunofluorescence: PFKP Antibody (OTI1D6) [NBP2-01539]

Immunocytochemistry/Immunofluorescence: PFKP Antibody (OTI1D6) [NBP2-01539] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP.
Immunohistochemistry-Paraffin: PFKP Antibody (OTI1D6) [NBP2-01539]

Immunohistochemistry-Paraffin: PFKP Antibody (OTI1D6) [NBP2-01539]

Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody.
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Applications for PFKP Antibody (OTI1D6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PFKP

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM].

Alternate Names

6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, EC 2.7.1, EC 2.7.1.11, PFK-CFLJ40226, PFKFplatelet type, Phosphofructokinase 1, phosphofructokinase, platelet, phosphohexokinase

Entrez Gene IDs

5214 (Human)

Gene Symbol

PFKP

Additional PFKP Products

Product Documents for PFKP Antibody (OTI1D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PFKP Antibody (OTI1D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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