PGAM1 Antibody (2G1-A6)

Novus Biologicals, part of Bio-Techne | Catalog # H00005223-M01

Novus Biologicals, part of Bio-Techne
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H00005223-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G1-A6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PGAM1 Antibodies »

Product Specifications

Immunogen

PGAM1 (AAH11678.1, 1 a.a. ~ 254 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVYELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK

Specificity

PGAM1 - phosphoglycerate mutase 1 (brain)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PGAM1 Antibody (2G1-A6)

Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01]

Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01]

Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01] - Analysis of PGAM1 expression in transfected 293T cell line by PGAM1 monoclonal antibody (M01), clone 2G1-A6.Lane 1: PGAM1 transfected lysate (Predicted MW: 28.8 KDa).Lane 2: Non-transfected lysate.
Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01]

Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01]

Western Blot: PGAM1 Antibody (2G1-A6) [H00005223-M01] - PGAM1 monoclonal antibody (M01), clone 2G1-A6 Analysis of PGAM1 expression in Jurkat.
ELISA: PGAM1 Antibody (2G1-A6) [H00005223-M01]

ELISA: PGAM1 Antibody (2G1-A6) [H00005223-M01]

ELISA: PGAM1 Antibody (2G1-A6) [H00005223-M01] - Detection limit for recombinant GST tagged PGAM1 is approximately 0.03ng/ml as a capture antibody.

Applications for PGAM1 Antibody (2G1-A6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGAM1

PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.

Alternate Names

BPG-dependent PGAM 1, EC 3.1.3.13, EC 5.4.2.1, EC 5.4.2.4, PGAMA, PGAM-Bphosphoglycerate mutase 1, phosphoglycerate mutase 1 (brain), phosphoglycerate mutase A, nonmuscle form, Phosphoglycerate mutase isozyme B

Entrez Gene IDs

5223 (Human)

Gene Symbol

PGAM1

OMIM

172250 (Human)

UniProt

AAH11678 (Human)

Additional PGAM1 Products

Product Documents for PGAM1 Antibody (2G1-A6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PGAM1 Antibody (2G1-A6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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