PGAM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46676

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-46676
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PGAM1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPMEPDHPFYSNISKDRRYADLTEDQLPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGAM1 Antibody

Western Blot: PGAM1 Antibody [NBP2-46676]

Western Blot: PGAM1 Antibody [NBP2-46676]

Western Blot: PGAM1 Antibody [NBP2-46676] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Liver tissue, Lane 6: Human Tonsil tissue
Immunocytochemistry/ Immunofluorescence: PGAM1 Antibody [NBP2-46676]

Immunocytochemistry/ Immunofluorescence: PGAM1 Antibody [NBP2-46676]

Immunocytochemistry/Immunofluorescence: PGAM1 Antibody [NBP2-46676] - Staining of human cell line PC-3 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PGAM1 Antibody [NBP2-46676]

Immunohistochemistry-Paraffin: PGAM1 Antibody [NBP2-46676]

Immunohistochemistry-Paraffin: PGAM1 Antibody [NBP2-46676] - Staining of human adrenal gland shows moderate positivity in glandular cells.

Applications for PGAM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGAM1

PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.

Alternate Names

BPG-dependent PGAM 1, EC 3.1.3.13, EC 5.4.2.1, EC 5.4.2.4, PGAMA, PGAM-Bphosphoglycerate mutase 1, phosphoglycerate mutase 1 (brain), phosphoglycerate mutase A, nonmuscle form, Phosphoglycerate mutase isozyme B

Entrez Gene IDs

5223 (Human)

Gene Symbol

PGAM1

UniProt

P18669 (Human)

Additional PGAM1 Products

Product Documents for PGAM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PGAM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.