PGK1 Antibody (2H4)
Novus Biologicals, part of Bio-Techne | Catalog # H00005230-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2H4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PGK1 (NP_000282, 321 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI
Specificity
PGK1 - phosphoglycerate kinase 1 (2H4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PGK1 Antibody (2H4)
![Western Blot: PGK1 Antibody (2H4) [H00005230-M02]](https://resources.bio-techne.com/images/products/PGK1-Antibody-2H4-Western-Blot-H00005230-M02-img0004.jpg "Western Blot: PGK1 Antibody (2H4) [H00005230-M02]")
Western Blot: PGK1 Antibody (2H4) [H00005230-M02]
Western Blot: PGK1 Antibody (2H4) [H00005230-M02] - PGK1 monoclonal antibody (M02), clone 2H4. Analysis of PGK1 expression in HepG2.
Sandwich ELISA: PGK1 Antibody (2H4) [H00005230-M02] - Detection limit for recombinant GST tagged PGK1 is approximately 3ng/ml as a capture antibody.
Applications for PGK1 Antibody (2H4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PGK1
Long Name
Phosphoglycerate Kinase-1
Alternate Names
MIG10, PGKA, PRP2
Entrez Gene IDs
5230 (Human)
Gene Symbol
PGK1
UniProt
Additional PGK1 Products
Product Documents for PGK1 Antibody (2H4)
Product Specific Notices for PGK1 Antibody (2H4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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