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PGK1 Antibody (2H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005230-M02

Novus Biologicals, part of Bio-Techne
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H00005230-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PGK1 (NP_000282, 321 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

Specificity

PGK1 - phosphoglycerate kinase 1 (2H4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PGK1 Antibody (2H4)

Western Blot: PGK1 Antibody (2H4) [H00005230-M02]

Western Blot: PGK1 Antibody (2H4) [H00005230-M02]

Western Blot: PGK1 Antibody (2H4) [H00005230-M02] - PGK1 monoclonal antibody (M02), clone 2H4. Analysis of PGK1 expression in HepG2.
Sandwich ELISA: PGK1 Antibody (2H4) [H00005230-M02] - Detection limit for recombinant GST tagged PGK1 is approximately 3ng/ml as a capture antibody.

Applications for PGK1 Antibody (2H4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGK1

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. A pseudogene of this gene has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Long Name

Phosphoglycerate Kinase-1

Alternate Names

MIG10, PGKA, PRP2

Entrez Gene IDs

5230 (Human)

Gene Symbol

PGK1

Additional PGK1 Products

Product Documents for PGK1 Antibody (2H4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGK1 Antibody (2H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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