PGK1 Antibody (GT21612)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13618
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (96%), Chimpanzee (100%), Mouse (97%), Porcine (96%), Rhesus Macaque (100%), Sheep (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT21612
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for PGK1 Antibody (GT21612)
Immunogen
Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (87%), Xenopus laevis (84%), Chicken (88%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for PGK1 Antibody (GT21612)
![Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]](https://resources.bio-techne.com/images/products/PGK1-Antibody-GT21612-Western-Blot-NBP3-13618-img0003.jpg "Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]")
Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]
Western Blot: PGK1 Antibody (GT21612) [NBP3-13618] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618]](https://resources.bio-techne.com/images/products/PGK1-Antibody-GT21612-Immunohistochemistry-Paraffin-NBP3-13618-img0002.jpg "Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618]")
Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618]
Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618] - PGK1 antibody [GT21612] detects PGK1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PGK1 stained by PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]](https://resources.bio-techne.com/images/products/PGK1-Antibody-GT21612-Western-Blot-NBP3-13618-img0001.jpg "Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]")
Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]
Western Blot: PGK1 Antibody (GT21612) [NBP3-13618] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Applications for PGK1 Antibody (GT21612)
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PGK1
Long Name
Phosphoglycerate Kinase-1
Alternate Names
MIG10, PGKA, PRP2
Gene Symbol
PGK1
Additional PGK1 Products
Product Documents for PGK1 Antibody (GT21612)
Product Specific Notices for PGK1 Antibody (GT21612)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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