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PGLS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47334

Novus Biologicals, part of Bio-Techne
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NBP2-47334

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IVAPISDSPKPPPQRVTLTLPVLNAARTVIFVATGEGKAAVLKRILEDQEENPLPAALVQPHTGKLCWFL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGLS Antibody

Western Blot: PGLS Antibody [NBP2-47334]

Western Blot: PGLS Antibody [NBP2-47334]

Western Blot: PGLS Antibody [NBP2-47334] - Analysis using Anti-PGLS antibody NBP2-47334 (A) shows similar pattern to independent antibody NBP2-47333 (B).
Immunocytochemistry/ Immunofluorescence: PGLS Antibody [NBP2-47334]

Immunocytochemistry/ Immunofluorescence: PGLS Antibody [NBP2-47334]

Immunocytochemistry/Immunofluorescence: PGLS Antibody [NBP2-47334] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
Immunohistochemistry: PGLS Antibody [NBP2-47334]

Immunohistochemistry: PGLS Antibody [NBP2-47334]

Immunohistochemistry: PGLS Antibody [NBP2-47334] - Staining of human bronchus shows strong positivity in respiratory epithelial cells.

Applications for PGLS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGLS

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate

Alternate Names

6PGLEC 3.1.1.31, 6-phosphogluconolactonase

Gene Symbol

PGLS

Additional PGLS Products

Product Documents for PGLS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGLS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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