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PGLYRP3/PGRPIA Antibody (187C434)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56729

Novus Biologicals, part of Bio-Techne
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NB100-56729
NB100-56729SS

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry Control, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 187C434

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 178-193 of human PGRP-1a.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for PGLYRP3/PGRPIA Antibody (187C434)

Western Blot: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729]

Western Blot: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729]

Western Blot: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729] - Analysis in cell lysates from human brain using this antibody at a dilution of 1:500.
Immunocytochemistry/ Immunofluorescence: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729]

Immunocytochemistry/ Immunofluorescence: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729]

Immunocytochemistry/Immunofluorescence: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729] - A. HCECs were exposed to HKCA (106 cells/ml) with prior incubation in the absence or presence of isotype IgG (10ug/ml), dectin-1 neutralizing Ab (10ug/ml), BAY11-7082 (10uM) or NF-kB activation inhibitor quinazoline (NF-kB-I, 10uM) for 1 h. HCECs were treated with 106 cells/ml HKCA for 48 hours in 8-chamber slides and examined by immunofluorescent staining for PGLYRPs 3 and 4. (NB100-56721) C. The percentages of positive cells of PGLYRPs 3 and 4 staining in HCECs in A was quantified. Results shown are the mean +/- SD of four independent experiments; *** p<0.001, as compared with normal control; ^^ p<0.005, ^^^ p<0.001, as compared with HCECs exposed to HKCA. Magnification: 400x (bar = 25um). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0128039) licensed under a CC-BY license.
PGLYRP3/PGRPIA Antibody (187C434)

Immunohistochemistry: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729] -

NF-kappaB p65 activation was induced by HKCA and inhibited by dectin-1 neutralizing antibody and NF-kappaB activation inhibitor quinazoline (NF-kappaB-I) in HCECs.A). HCECs were exposed to HKCA (106 cells/ml) with prior incubation in the absence or presence of isotype IgG (10ug/ml), dectin-1 neutralizing Ab (10ug/ml), BAY11-7082 (10uM) or NF-kappaB activation inhibitor quinazoline (NF-kappaB-I, 10uM) for 1 h. HCECs were treated with 106 cells/ml HKCA for 48 hours in 8-chamber slides and examined by immunofluorescent staining for PGLYRPs 2-4. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26039076), licensed under a CC-BY licence.

Applications for PGLYRP3/PGRPIA Antibody (187C434)

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 15839897)

Flow (Intracellular)

reported in scientific literature (PMID 16849490)

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (Uehara et al (2005))

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGLYRP3/PGRP-I alpha

Primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-1a (PGRP-3), and PGRP-1beta (PGRP-4)) define a new family of human pattern recognition molecules. PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood. PGRP-1a mRNA is highly expressed in esophagus, tonsils and thymus and to lesser extent in the stomach, rectum, and brain.

Long Name

Peptidoglycan Recognition Protein Intermediate, Alpha/Peptidoglycan Recognition Protein 3

Alternate Names

PGRP-I alpha, PGRP1A, PGRPI alpha, PGRPIA

Gene Symbol

PGLYRP3

UniProt

Additional PGLYRP3/PGRP-I alpha Products

Product Documents for PGLYRP3/PGRPIA Antibody (187C434)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGLYRP3/PGRPIA Antibody (187C434)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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