PGM1 Antibody (3B8-H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005236-M01

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
H00005236-M01
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Bovine

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B8-H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PGM1 Antibodies »

Product Specifications

Immunogen

PGM1 (AAH19920, 1 a.a. ~ 562 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVKIVTVKTQAYQDQKPGTSGLRKRVKVFQSSANYAENFIQSIISTVEPAQRQEATLVVGGDGRFYMKEAIQLIARIAAANGIGRLVIGQNGILSTPAVSCIIRKIKAIGGIILTASHNPGGPNGDFGIKFNISNGGPAPEAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQFDLENKFKPFTVEIVDSVEAYATMLRSIFDFSALKELLSGPNRLKIRIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKYGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT

Specificity

PGM1 - phosphoglucomutase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PGM1 Antibody (3B8-H4)

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01]

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01]

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01]

Immunocytochemistry/ Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01]

Immunocytochemistry/Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of monoclonal antibody to PGM1 on HeLa cell. Antibody concentration 20 ug/ml.
Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01]

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01]

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( Cat # H00005236-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody (M01), clone 3B8-H4 (Cat # H00005236-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
View 3 more images

Applications for PGM1 Antibody (3B8-H4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGM1

Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 (PubMed 8257433)).(supplied by OMIM)

Alternate Names

EC 5.4.2, EC 5.4.2.2, Glucose phosphomutase 1, GSD14, PGM 1, phosphoglucomutase 1, phosphoglucomutase-1

Entrez Gene IDs

5236 (Human)

Gene Symbol

PGM1

OMIM

171900 (Human)

UniProt

AAH19920 (Human)

Additional PGM1 Products

Product Documents for PGM1 Antibody (3B8-H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PGM1 Antibody (3B8-H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.