PGM1 Antibody (CL3299)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61618

Novus Biologicals, part of Bio-Techne
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NBP2-61618
NBP2-61618-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3299

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PGM1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SILATRKQSVEDILKDHWQKHGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSD

Epitope

HGRNFFTRYD

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PGM1 Antibody (CL3299)

Western Blot: PGM1 Antibody (CL3299) [NBP2-61618]

Western Blot: PGM1 Antibody (CL3299) [NBP2-61618]

Western Blot: PGM1 Antibody (CL3299) [NBP2-61618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: PGM1 Antibody (CL3299) [NBP2-61618]

Immunohistochemistry-Paraffin: PGM1 Antibody (CL3299) [NBP2-61618]

Immunohistochemistry-Paraffin: PGM1 Antibody (CL3299) [NBP2-61618] - Staining of human fallopian tube shows absence of immunoreactivity (negative control).
Immunohistochemistry: PGM1 Antibody (CL3299) [NBP2-61618]

Immunohistochemistry: PGM1 Antibody (CL3299) [NBP2-61618]

Immunohistochemistry: PGM1 Antibody (CL3299) [NBP2-61618] - Staining of human skeletal muscle shows moderate cytoplasmic immunoreactivity in muscle fibers.
View 3 more images

Applications for PGM1 Antibody (CL3299)

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGM1

Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 (PubMed 8257433)).(supplied by OMIM)

Alternate Names

EC 5.4.2, EC 5.4.2.2, Glucose phosphomutase 1, GSD14, PGM 1, phosphoglucomutase 1, phosphoglucomutase-1

Gene Symbol

PGM1

Additional PGM1 Products

Product Documents for PGM1 Antibody (CL3299)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PGM1 Antibody (CL3299)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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