PGM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85982

Novus Biologicals, part of Bio-Techne
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NBP1-85982
NBP1-85982-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PGM1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGM1 Antibody

Western Blot: PGM1 Antibody [NBP1-85982]

Western Blot: PGM1 Antibody [NBP1-85982]

Western Blot: PGM1 Antibody [NBP1-85982] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: PGM1 Antibody [NBP1-85982]

Immunocytochemistry/ Immunofluorescence: PGM1 Antibody [NBP1-85982]

Immunocytochemistry/Immunofluorescence: PGM1 Antibody [NBP1-85982] - Immunofluorescent staining of human cell line SiHa shows localization to actin filaments.
Immunohistochemistry-Paraffin: PGM1 Antibody [NBP1-85982]

Immunohistochemistry-Paraffin: PGM1 Antibody [NBP1-85982]

Immunohistochemistry-Paraffin: PGM1 Antibody [NBP1-85982] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
View 1 more images

Applications for PGM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGM1

Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 (PubMed 8257433)).(supplied by OMIM)

Alternate Names

EC 5.4.2, EC 5.4.2.2, Glucose phosphomutase 1, GSD14, PGM 1, phosphoglucomutase 1, phosphoglucomutase-1

Gene Symbol

PGM1

Additional PGM1 Products

Product Documents for PGM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PGM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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