Key Product Details

Species Reactivity

Human, Rat, Mouse (Negative)

Applications

Electron Microscopy, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PHD4/HIF Prolyl Hydroxylase 4 Antibodies »

Product Specifications

Immunogen

The epitope recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 502) of human PHD4/HIF Prolyl Hydroxylase 4 using the numbering given in entry NP_808808.1 (GeneID 54681).

Reactivity Notes

Does not appear to work in Mouse. Rat reactivity reported in scientific literature (PMID: 17003483)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for PHD4/HIF Prolyl Hydroxylase 4 Antibody [Biotin]

Application
Recommended Usage

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This PHD4/HIF Prolyl Hydroxylase 4 antibody is useful for Western Blot where a band is seen at ~50 kDa. Use in Immunocytochemistry/immunofluorescence, Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: PHD4/HIF Prolyl Hydroxylase 4

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Alternate Names

EC 1.14.11.-, EGLN4, HIFPH4, HIF-PH4, HIF-prolyl hydroxylase 4, HPH-4, hypoxia-inducible factor prolyl 4-hydroxylase, Hypoxia-inducible factor prolyl hydroxylase 4, P4H with transmembrane domain, P4H-TMFLJ20262, PH-4, PH4 hypoxia inducible factor prolyl 4 hydroxylase, PHD4, proline 4-hydroxylase, prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum), Prolyl hydroxlase domain-containing 4, transmembrane prolyl 4-hydroxylase

Gene Symbol

P4HTM

Additional PHD4/HIF Prolyl Hydroxylase 4 Products

Product Documents for PHD4/HIF Prolyl Hydroxylase 4 Antibody [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PHD4/HIF Prolyl Hydroxylase 4 Antibody [Biotin]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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