PHF10 Antibody (PCRP-PHF10-2A10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13750

Novus Biologicals, part of Bio-Techne
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NBP3-13750-100ug
NBP3-13750-20ug
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-PHF10-2A10

Concentration

0.2 mg/ml

View all PHF10 Antibodies »

Product Specifications

Immunogen

Recombinant full-length humanPHF10protein (Uniprot: Q8WUB8)

Reactivity Notes

Predicted to react in Mouse and Chick.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

56.05 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14153)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PHF10 Antibody (PCRP-PHF10-2A10)

Immunocytochemistry/ Immunofluorescence: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750]

Immunocytochemistry/ Immunofluorescence: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750]

Immunocytochemistry/Immunofluorescence: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Antibody (PCRP-PHF10-2A10) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750]

Flow Cytometry: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750]

Flow Cytometry: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750] - Flow cytometric analysis of PFA-fixed HeLa cells. PHF10 antibody (PCRP-PHF10-2A10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750]

Protein Array: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750]

Protein Array: PHF10 Antibody (PCRP-PHF10-2A10) [NBP3-13750] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PHF10 Antibody (PCRP-PHF10-2A10).

Applications for PHF10 Antibody (PCRP-PHF10-2A10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PHF10

PHF10 contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq]

Alternate Names

BAF45a, BAF45APHD zinc finger protein XAP135, BRG1-associated factor 45a, FLJ10975, MGC111009, PHD finger protein 10, XAP135BRG1-associated factor, 45-KD, A

Gene Symbol

PHF10

UniProt

Q8WUB8 (Human)

Additional PHF10 Products

Product Documents for PHF10 Antibody (PCRP-PHF10-2A10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHF10 Antibody (PCRP-PHF10-2A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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