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PHF11 Antibody (3B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00051131-M01

Novus Biologicals, part of Bio-Techne
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H00051131-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PHF11 (AAH17212, 1 a.a. ~ 292 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKRTCALCPKDVEYNVLYFAQSENIAAHENCLLYSSGLVECEDQDPLNPDRSFDVESVKKEIQRGRKLKCKFCHKRGATVGCDLKNCNKNYHFFCAKKDDAVPQSDGVRGIYKLLCQQHAQFPIIAQSAKFSGVKRKRGRKKPLSGNHVQPPETMKCNTFIRQVKEEHGRHTDATVKVPFLKKCKEAGLLNYLLEEILDKVHSIPEKLMDETTSESDYEEIGSALFDCRLFEDTFVNFQAAIEKKIHASQQRWQQLKEEIELLQDLKQTLCSFQENRDLMSSSTSISSLSY

Specificity

Reacts with PHD finger protein 11.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PHF11 Antibody (3B8)

Western Blot: PHF11 Antibody (3B8) [H00051131-M01]

Western Blot: PHF11 Antibody (3B8) [H00051131-M01]

Western Blot: PHF11 Antibody (3B8) [H00051131-M01] - Analysis of PHF11 expression in transfected 293T cell line by PHF11 monoclonal antibody (M01), clone 3B8.Lane 1: PHF11 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PHF11 Antibody (3B8) [H00051131-M01]

Immunocytochemistry/ Immunofluorescence: PHF11 Antibody (3B8) [H00051131-M01]

Immunocytochemistry/Immunofluorescence: PHF11 Antibody (3B8) [H00051131-M01] - Analysis of monoclonal antibody to PHF11 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: PHF11 Antibody (3B8) [H00051131-M01] - Detection limit for recombinant GST tagged PHF11 is 3 ng/ml as a capture antibody.

Applications for PHF11 Antibody (3B8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHF11

These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognize native protein in any application.

Alternate Names

APY, BCAPIGHER, BRCA1 C-terminus-associated protein, IgE responsiveness (atopic), IGEL, IGER, NYREN34, NY-REN-34, NY-REN-34 antigen, PHD finger protein 11, Renal carcinoma antigen NY-REN-34

Entrez Gene IDs

51131 (Human)

Gene Symbol

PHF11

Additional PHF11 Products

Product Documents for PHF11 Antibody (3B8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHF11 Antibody (3B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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