PHF15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81531

Novus Biologicals, part of Bio-Techne
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NBP1-81531
NBP1-81531-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHF15 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASVAADSDVQVLGPAASPKPLGRLRPPRESKVTRRLPGARPDAGMGPPSAVAERPKVSLHFDTETDGYFSDGEMSDSDVEAEDGGVQRGPREAGAEEVVRMGVLA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF15 Antibody

Immunocytochemistry/ Immunofluorescence: PHF15 Antibody [NBP1-81531]

Immunocytochemistry/ Immunofluorescence: PHF15 Antibody [NBP1-81531]

Immunocytochemistry/Immunofluorescence: PHF15 Antibody [NBP1-81531] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PHF15 Antibody [NBP1-81531]

Immunohistochemistry-Paraffin: PHF15 Antibody [NBP1-81531]

Immunohistochemistry-Paraffin: PHF15 Antibody [NBP1-81531] - Staining of human tonsil shows moderate nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: PHF15 Antibody [NBP1-81531]

Immunohistochemistry-Paraffin: PHF15 Antibody [NBP1-81531]

Immunohistochemistry-Paraffin: PHF15 Antibody [NBP1-81531] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
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Applications for PHF15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHF15

PHF15, also known as Protein Jade-2, has 3 isoforms, a 790 amino acid isoform that is 87 kDa, a 789 amino acid isoform that is 87 kDa and a 562 amino acid isoform that is 63 kDa, has a histone H4-specific acetyltransferase activity as a component of the HBO1complex, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. This protein has been shown an interaction with ING4, KAT7, ING5, PACSIN, ZBTB3, ZBTB5, ZNF496, and VHL proteins in histone H3 acetylation, histone H4-K5 acetylation, histone H4-K8 acetylation, and histone H4-K12 acetylation biological processes.

Alternate Names

PHD finger protein 15KIAA0239JADE2, protein Jade-2

Gene Symbol

PHF15

Additional PHF15 Products

Product Documents for PHF15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHF15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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